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Jefferson de Guzman, M.S.

Jefferson de Guzman, M.S.IDP, University of Florida

Ph.D. Student

Education

  • M.S. in Medical Science, Kyungpook National University School of Medicine, South Korea 2003-2005
  • Thesis: Molecular characterization of two myoglobins of the lung fluke, Paragonimus westermani
  • B.S. in Public Health, University of the Philippines, Manila, Philippines 1998-2002
  • Thesis: Process evaluation of a campus-based reproductive health program in a university in the Philippines.

Current and Previous Research Positions

Research Scientist — 2009 to present
Department of Periodontology College of Dentistry and Emerging Pathogens Institute
University of Florida, Gainesville, Fla., USA
Graduate Research Assistant — 2003 to 2005
Department of Parasitology, School of Medicine
Kyungpook National University, South Korea
Research Assistant — 2002 to 2003
Department of Parasitology, College of Public Health
University of the Philippines Manila, Philippines

Current Research Work

  • Characterization of adenosine A2a receptor in human primary gingival epithelial cells and its role in Porphyromonas gingivalis infection
  • Intracellular trafficking of Porphyromonas gingivalis in primary gingival epithelial cells
  • Modulation of host reactive oxygen species by Porphyromonas gingivalis

Past Research Work

  • Subcellular localization of the Outer Membrane Protein 38 of Acinetobacter baumannii human epithelial cells
  • Comparison of gene expression of the diploid and triploid Paragonimus westermani adults by expressed sequence tag analysis
  • Molecular characterization of a gynecophoral canal protein of Paragonimus westermani
  • Identification and characterization of the fascilin I domain-containing protein of Paragonimus westermaniMolecular characterization of a cDNA encoding actin-binding protein of Acanthamoeba healyi
  • Evaluation of the pilot testing of rapid diagnostic tests for malaria, application and performance of the clinical algorithm for diagnosis of malaria and pilot testing of the certification program for quality assurance microscopy in a malaria endemic region in the Philippines
  • Situational analysis and development of a health educational program in a rural community in the Philippines

Research Techniques

Laboratory animal techniques
Intravenous injection of recombinant proteins in rats, polyclonal antibody generation in rats
Molecular biology techniques
cDNA library synthesis, DNA cloning and amplification by PCR, gel electrophoresis, recombinant protein production, immunohistochemistry, DNA, RNA and protein purification, ELISA, bacterial and mammalian cell culture, primary cell culture, bacterial transformation and conjugation, plasmid construct cloning and extraction, Northern, Western and Southern blots, Quantitative PCR, gene expression silencing by siRNA
Fluorescence microscopy, confocal microscopy, differential interference contrast microscopy, electron microscopy

Publications

Peer-reviewed research papers:
  • Jefferson V. de Guzman, Chul Hee Choi, David M. Ojcius, and Özlem Yilmaz. “Danger signal adenosine stimulates growth of Porphyromonas gingivalis in primary gingival epithelial cells.” (Manuscript in submission)
  • Chul Hee Choi, Jefferson V. de Guzman, Richard J. Lamont, and Özlem Yilmaz. “Genetic transformation of an obligate anaerobe, P. gingivalis for FMN-green fluorescent protein expression in studying host-microbe interaction. PLoS ONE 6(4): e18499, 2011.
  • Su-Min Song, Jong-Won Shin, Jefferson V. de Guzman, Jin Kim, Hak-Sun Yu, Bijay Kumar Jha, Hyun-Hee Kong, Yeonchul Hong, and Dong-Il Chung. “Paragonimus westermani: identification and characterization of the fascilin 1 domain-containing protein.” Experimental Parasitology 125(2):76-83, 2010.
  • Jefferson V. de Guzman, Hak-Sun Yu, Hae-Jin Jeong, Yeon-Chul Hong, Jin Kim, Hyun-Hee Kong, and Dong-Il Chung. “Molecular characterization of two myoglobins of Paragonimus westermani.” Journal of Parasitology 93(1):97-103, 2007.
  • Tae-Suk Kim, Jefferson V. de Guzman, Hyun-Hee Kong, and Dong-Il Chung. “Comparison of gene representation between diploid and triploid Paragonimus westermani by expressed sequence tag analysis.” Journal of Parasitology 92(4):803-816, 2006.
Peer-reviewed abstracts
Oral Presentations:
  • Jefferson V. de Guzman, Chul Hee Choi, David M. Ojcius, and Özlem Yilmaz. Danger signal adenosine stimulates growth of Porphyromonas gingivalis in primary gingival epithelial cells. (2011, International Association for Dental Research General Session and Exhibition, San Diego, CA, USA)
  • Chul Hee Choi, Jefferson V. de Guzman, David M. Ojcius, and Özlem Yilmaz. Modulation of intracellular reactive-oxygen-species in primary gingival epithelial cells by Porphyromonas infection. (2011, International Association for Dental Research General Session and Exhibition, San Diego, CA, USA; Session Chair)
  • Jefferson V. de Guzman, Chul Hee Choi, Matthew Pettengill, David M. Ojcius, and Özlem Yilmaz. Danger signal adenosine attributes to the persistence ofPorphyromonas gingivalis in human primary gingival epithelial cells. (2010, International Association for Dental Research General Session and Exhibition, Barcelona, Spain)
  • Jefferson V. de Guzman, Hak-Sun Yu, Hyun-Hee Kong, and Dong-Il Chung. Molecular characterization of two myoglobins of Paragonimus westermani. (2005, Korean Society for Parasitology Annual Meeting, South Korea)
  • Jefferson V. de Guzman, Hyun-Hee Kong, Dong-Il Chung. Expressed sequence tag analysis of the triploid Paragonimus westermani adults. (2004, Korean Society for Parasitology Annual Meeting, South Korea)
Poster Presentations:
  • Jefferson V. de Guzman, Chul Hee Choi, David M. Ojcius, and Özlem Yilmaz. Danger signal adenosine stimulates growth of Porphyromonas gingivalis in primary gingival epithelial cells. (2011, American Society for Microbiologists General Meeting, New Orleans, LA, USA)
  • Chul Hee Choi, Jefferson V. de Guzman, David M. Ojcius, and Özlem Yilmaz. Modulation of intracellular reactive-oxygen-species in primary gingival epithelial cells by nucleoside diphosphate kinase of Porphyromonas gingivalis. (2011, American Society for Microbiologists General Meeting, New Orleans, LA, USA)
  • Chul Hee Choi, Ralee Spooner, Jefferson V. de Guzman, and Özlem Yilmaz. Inhibiting production of reactive-oxygen-species in primary gingival epithelial cells by Porphyromonas gingivalis. (2010, International Association for Dental Research General Session and Exhibition, Barcelona, Spain)
  • Joanna I. Alafag, Eun-Kyung Moon, Jefferson V. de Guzman, Dong-Il Chung, and Hyun-Hee Kong. Molecular cloning and biochemical characterization of a novel actin bundling protein in Acanthamoeba healyi. (2005, Korean Society for Parasitology Annual Meeting, South Korea)
Other presentations
  • Jefferson V. de Guzman, Chul Hee Choi, David M. Ojcius, and Özlem Yilmaz. Danger signal adenosine stimulates growth of Porphyromonas gingivalis in primary gingival epithelial cells. (2011, Emerging Pathogens Institute Annual Research Day, University of Florida, USA)
  • Chul Hee Choi, Jefferson V. de Guzman, David M. Ojcius, and Özlem Yilmaz. Modulation of intracellular reactive-oxygen-species in primary gingival epithelial cells by nucleoside diphosphate kinase of Porphyromonas gingivalis. (2011, Spring Synergy Celebration of Science, College of Dentistry, University of Florida, USA)
  • Chul Hee Choi, Jefferson V. de Guzman, Luyu Yao, Ralee Spooner, David M. Ojcius, and Özlem Yilmaz. ATP-dependent activation of an inflammasome in primary gingival epithelial cells infected by periodontal pathogenPorphyromonas gingivalis. (2010, Emerging Pathogens Institute Annual Research Day, University of Florida, USA)
  • Chul Hee Choi, Ralee Spooner, Luyu Yao, Jefferson V. de Guzman, and Özlem Yilmaz. Modulation of extracellular ATP-induced gingival epithelial cell death by Porphyromonas gingivalis. (2009, Interdisciplinary Research Exhibition on Infectious Diseases, University of Florida, USA)